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A flow injection-fluorometric method for the determination of ammonium in fresh and saline waters with a view to in situ analyses ArchiMer
Aminot, Alain; Kerouel, Roger; Birot, Dominique.
A version of the orthophthaldialdehyde-fluorescence ammonium determination for flow injection analysis (FIA) is presented here, with a view to its use for in situ, low-power consumption systems, Thus, the reaction temperature was limited to 30 degreesC and FIA was used in stop-Bow mode (3 min stop). The calibration is linear up to 50 mu mol l(-1), but concentrations up to 100 mu mol l(-1) can be measured. Repeatability is around 1% in the range of 0.5-4 mu mol l(-1) and the detection limit is about 0.03 mu mol l(-1) Over the salinity range of 5-35 (seawater practical salinity scale) the salt effect is almost negligible (within +/- 2%); and below salinity of 5 it increases to a maximum of -9% in fresh water compared to seawater. Hydrogen carbonate,...
Tipo: Text Palavras-chave: In situ; Fluorometry; Flow injection analysis; Determination; Ammonium.
Ano: 2001 URL: http://archimer.ifremer.fr/doc/2001/publication-1722.pdf
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Advancing Observation of Ocean Biogeochemistry, Biology, and Ecosystems With Cost-Effective in situ Sensing Technologies ArchiMer
Wang, Zhaohui Aleck; Moustahfid, Hassan; Mueller, Amy V.; Michel, Anna Pm; Mowlem, Matthew; Glazer, Brian T.; Mooney, T. Aran; Michaels, William; Mcquillan, Jonathan S.; Robidart, Julie C.; Churchill, James; Sourisseau, Marc; Daniel, Anne; Schaap, Allison; Monk, Sam; Friedman, Kim; Brehmer, Patrice.
Advancing our understanding of ocean biogeochemistry, biology, and ecosystems relies on the ability to make observations both in the ocean and at the critical boundaries between the ocean and other earth systems at relevant spatial and temporal scales. After decades of advancement in ocean observing technologies, one of the key remaining challenges is how to cost-effectively make measurements at the increased resolution necessary for illuminating complex system processes and rapidly evolving changes. In recent years, biogeochemical in situ sensors have been emerging that are threefold or more lower in cost than established technologies; the cost reduction for many biological in situ sensors has also been significant, although the absolute costs are still...
Tipo: Text Palavras-chave: In situ; Sensor; OceanObs; Ocean technology; EOVs; Biogeochemistry; Biology; Cost effective.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00514/62567/66921.pdf
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Análise por hibridização in situ da expressão de metalotioneínas em Arachis stenosperma após inoculação com Meloigogyne arenaria (Nematoda). Infoteca-e
SILVA, P. Í. T.; RIBEIRO, S.; PROITE, K.; GUIMARÃES, P. M.; LEAL-BERTIOLI, S. C. M.; BERTIOLI, D.; CARNEIRO, R.; ARAÚJO, A. C. G..
bitstream/CENARGEN-2009-09/31574/3/bp223_1208.pdf
Tipo: Boletim de Pesquisa e Desenvolvimento (INFOTECA-E) Palavras-chave: Arachis stenosperma; Metalotioneínas; Análise da expressão; Hibridização; In situ; Meloigogyne arenaria (Nematoda); Melhoramento genético.; Amendoim; Controle Biológico; Inoculação; Nematóide..
Ano: 2008 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/191223
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Avaliação de métodos laboratoriais para estimar a digestibilidade e o valor energético de dietas para ruminantes Arq. Bras. Med. Vet. Zootec.
Silveira,M.F.; Kozloski,G.V.; Mesquita,F.R.; Farenzena,R.; Senger,C.C.D.; Brondani,I.L..
Foi avaliada a acurácia dos métodos laboratoriais para estimar a digestibilidade e o valor energético de dietas para bovinos de corte. As dietas experimentais foram isonitrogenadas e compostas por silagem de milho e 25, 40, 55 ou 70% de concentrado. Os valores de digestibilidade medidos in vivo foram comparados com os obtidos nos ensaios in situ, in vitro e in vitro/gases, e com valores estimados a partir de equações matemáticas baseadas na composição química das dietas. O ensaio in vivo foi realizado com quatro bovinos em delineamento em quadrado latino 4×4. Quatro animais foram usados para o ensaio in situ e quatro corridas foram realizadas para os ensaios in vitro. Não houve interação significativa de dietas versus métodos. As taxas de degradação...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ruminante; Digestibilidade; Equações; In situ; In vitro; In vivo.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352009000200021
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Conservação in situ de pequi em um Cerrado sensu stricto na terra indígena Krahô. Infoteca-e
MINERVINO, J. S.; DIAS, T. A. B.; KRAHÔ, A..
bitstream/CENARGEN-2009-09/31523/3/cot174_0408.pdf
Tipo: Comunicado Técnico (INFOTECA-E) Palavras-chave: In situ; Krahô; Recursos Genéticos; Conservation; Savana.; Conservação; Cerrado; Pequi.; Genetic resources..
Ano: 2008 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/191009
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Degradabilidade ruminal da fibra das frações do resíduo industrial de tomate Arq. Bras. Med. Vet. Zootec.
Campos,W.E.; Borges,A.L.C.C.; Saturnino,H.M.; Silva,R.R.; Sousa,B.M.; Rogério,M.C.P.; Borges,I.; Rodríguez,N.M..
Avaliou-se a degradabilidade in situ da fibra em detergente neutro (FDN) e da fibra em detergente ácido (FDA) das cascas e das sementes inteiras ou moídas (peneira de 2mm) do resíduo industrial de tomate (RIT). Foram utilizados três bovinos adultos, machos, em delineamento de blocos ao acaso com parcelas subdivididas. A solubilidade da FDN apresentou baixos valores para todas as frações estudadas, sendo semelhante entre cascas (3%), sementes inteiras (5%) e resíduo (7%). Sementes moídas apresentaram maior solubilidade da FDN (20%). Os potenciais de degradação da FDN para sementes moídas, RIT, cascas e sementes inteiras foram de 78, 64, 36 e 33%, respectivamente. As taxas de degradação da FDN variaram de 4,4 a 14,8%/h para as diferentes frações do RIT,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Tomate; In situ; Casca; Semente; FDN; FDA.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352007000100031
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Degradabilidade ruminal da matéria seca e proteína bruta, e tempo de colonização microbiana de oleaginosas, utilizadas na alimentação de ovinos - doi: 10.4025/actascianimsci.v33i4.11388 Animal Sciences
Goes, Rafael Henrique de Tonissi e Buschinelli de; UFGD; Souza, Kennyson Alves de; UFGD; Nogueira, Katherini Aline Guimarães; UFGD; Pereira, Danielly de Faria; UFGD; Oliveira, Euclides Reuter de; UFGD; Brabes, Kelly Cristina da Silva; UFGD.
Avaliou-se a degradabilidade in situ, dos grãos de linhaça, canola, colza e milho, caroço de algodão, e dos concentrados casca e farelo de soja, em ovinos. Foram utilizados três animais da raça Santa Inês, fistulados e providos de cânulas ruminais, mantidos em baias individuais, recebendo diariamente capim picado e ração concentrada. Os alimentos foram incubados em ordem decrescente de 72, 48, 24, 18, 6, 3 e 0h. O teor de proteína dos alimentos avaliados foram 34,77; 30,07; 23,70; 10,64; 26,12; 14,65 e 56,90% para canola, colza, linhaça, milho, caroço de algodão, casca de soja e farelo de soja, respectivamente. A canola e a colza apresentaram baixa degradabilidade efetiva para a MS, com valor médio de 33,68%. O grão de linhaça apresentou degradabilidade...
Tipo: Pesquisa Palavras-chave: 5.04.03.02-8 composição centesimal; Colza; Linhaça; In situ chemical composition; Rapeseed; Linseed; In situ.
Ano: 2011 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/11388
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Digestibility and ruminal digestion kinetics of corn silage Arq. Bras. Med. Vet. Zootec.
Di Marco,O.N.; Aello,M.S.; Arias,S..
The in situ dry matter (DM) disappearance of corn silages in two maturity stages (milk grain and half milk line) of known in vivo and in vitro digestibility was determined, with the main purpose of comparing digestibility values with the ruminal disappearance at 24 and 48h of incubation. A secondary goal was the description of their ruminal digestion kinetics, from which the effective degradability was calculated at an assumed passage rate of 4%/h. Data of in vivo, in vitro and in situ degradability at 24 and 48-h were analyzed with a linear model that included as fixed effects the maturity and the methodology of evaluation, and the kinetic data were described by the exponential model of McDonald. There was a significant effect (P<0.05) of methodology...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Corn silage; In situ; In vitro and in vivo digestibility; Ruminal kinetics.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352005000200014
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From Observation to Information and Users: The Copernicus Marine Service Perspective ArchiMer
Le Traon, Pierre-yves; Reppucci, Antonio; Alvarez Fanjul, Enrique; Aouf, Lotfi; Behrens, Arno; Belmonte, Maria; Bentamy, Abderrahim; Bertino, Laurent; Brando, Vittorio Ernesto; Kreiner, Matilde Brandt; Benkiran, Mounir; Carval, Thierry; Ciliberti, Stefania A.; Claustre, Hervé; Clementi, Emanuela; Coppini, Giovanni; Cossarini, Gianpiero; De Alfonso Alonso-muñoyerro, Marta; Delamarche, Anne; Dibarboure, Gerald; Dinessen, Frode; Drevillon, Marie; Drillet, Yann; Faugere, Yannice; Fernández, Vicente; Fleming, Andrew; Garcia-hermosa, M. Isabel; Sotillo, Marcos García; Garric, Gilles; Gasparin, Florent; Giordan, Cedric; Gehlen, Marion; Gregoire, Marilaure L.; Guinehut, Stephanie; Hamon, Michel; Harris, Chris; Hernandez, Fabrice; Hinkler, Jørgen B.; Hoyer, Jacob; Karvonen, Juha; Kay, Susan; King, Robert; Lavergne, Thomas; Lemieux-dudon, Benedicte; Lima, Leonardo; Mao, Chongyuan; Martin, Matthew J; Masina, Simona; Melet, Angelique; Buongiorno Nardelli, Bruno; Nolan, Glenn; Pascual, Ananda; Pistoia, Jenny; Palazov, Atanas; Piolle, Jean-francois; Pujol, Marie Isabelle; Pequignet, Anne Christine; Peneva, Elisaveta; Pérez Gómez, Begoña; Petit De La Villeon, Loic; Pinardi, Nadia; Pisano, Andrea; Pouliquen, Sylvie; Reid, Rebecca; Remy, Elisabeth; Santoleri, Rosalia; Siddorn, John; She, Jun; Staneva, Joanna; Stoffelen, Ad; Tonani, Marina; Vandenbulcke, Luc; Von Schuckmann, Karina; Volpe, Gianluca; Wettre, Cecilie; Zacharioudaki, Anna.
The Copernicus Marine Environment Monitoring Service (CMEMS) provides regular and systematic reference information on the physical and biogeochemical ocean and sea-ice state for the global ocean and the European regional seas. CMEMS serves a wide range of users (more than 15,000 users are now registered to the service) and applications. Observations are a fundamental pillar of the CMEMS value-added chain that goes from observation to information and users. Observations are used by CMEMS Thematic Assembly Centres (TACs) to derive high-level data products and by CMEMS Monitoring and Forecasting Centres (MFCs) to validate and constrain their global and regional ocean analysis and forecasting systems. This paper presents an overview of CMEMS, its evolution,...
Tipo: Text Palavras-chave: Ocean; Observing systems; Satellite; In situ; Data assimilation; Services.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00498/60983/64389.pdf
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How the carotid body works: Different strategies and preparations to solve different problems Biol. Res.
ZAPATA,PATRICIO; LARRAÍN,CAROLINA.
This is a review of the different experimental approaches developed to solve the problems in our progress towards a comprehensive understanding of how arterial chemoreceptors operate. An analysis is performed of the bases, advantages and limits of the following preparations: studies of ventilatory reflexes originated from carotid bodies (CBs) in the entire animal; recordings of CB chemosensory discharges in situ; CB preparations perfused in situ; CB explants in oculo; CB explants in ovo; CB preparations incubated in vitro; CB preparations superfused in vitro; CB preparations perfused and superfused in vitro; CB tissue slices in vitro; cells acutely dissociated from CBs; CB cells in tissue culture; petrosal ganglia superfused in vitro; petrosal ganglion...
Tipo: Journal article Palavras-chave: Arterial chemoreceptors; Carotid body; Chemosensory activity; Co-cultures; Cultures; In situ; In vitro; Petrosal ganglia; Superfusion.
Ano: 2005 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000400002
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Increasing Reliability: Smart Biofouling Prevention Systems ArchiMer
Delauney, Laurent; Ralston, Emilie; Zargiel-hunsucker, Kelli.
Tipo: Text Palavras-chave: Biofilm; Biofouling; Camera; In situ; Lights; Long term; Marine; Monitoring; Optical; Protection; Sensors..
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00630/74233/73867.pdf
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Ruminal degradability of brown-midrib sorghum-sudangrass hybrids for cutting and grazing Rev. Ciênc. Agron.
Ferreira,Deborah Alves; Gonçalves,Lúcio Carlos; Rodrigues,José Avelino Santos.
ABSTRACT Greater ruminal degradability has been reported in mutant plants with brown midrib (bmr). The aim of this study was to evaluate the in situ degradability of dry matter (DM), neutral detergent fibre (NDF) and crude protein (CP) in the experimental sorghum-sudangrass bmr hybrids BR007 x Tx2784bmr and Tx635x Tx2785bmr, conventional experimental hybrids CMSxS206 x Tx2784 and Tx636 x Tx2785, and the control hybrid BRS801, harvested 51 days after planting. Three sheep with ruminal cannulae were used. The experimental design was of randomised complete blocks in subdivided lots. The mean values for DM and NDF disappearance were higher in the bmr hybrids after 6 h incubation. These plants had a higher rate of degradation (mean value of 6.93%/h) for...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Forage; In situ; Ruminants; Sorghum bicolor; Sorghum sudanense.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902018000100141
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Ruminal silage degradability and productivity of forage and grain-type sorghum cultivars Scientia Agricola
Resende,José Antônio; Pereira,Marcos Neves; Pinho,Renzo Garcia Von; Fonseca,Abeilard Henrique; Silva,Aloísio Ricardo Pereira da.
Forages for feeding dairy cows should conciliate the potential for high dry matter yield per unit area, maximizing animal stocking rate, with high nutritive value, which enables decreasing the amount of concentrate feedstuffs per milk unit produced. Eighteen sorghum cultivars were cultivated 0.70 m apart and with 12 plants per linear meter. Plants harvested at the milk-to-dough stage were ensiled in the laboratory. Eleven agronomic and chemical traits were evaluated. Silage samples were incubated in situ and the effective ruminal dry matter degradation (DEF) was calculated assuming a fractional passage rate of -0.04 h-1. Dry matter yield was 13.0 ± 2.3 (mean ± S.D.) t ha-1. The neutral detergent fiber content was 50.3 ± 3.8 and the DEF was 48.8 ± 2.8% (dry...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Digestibility; Fiber; Rumen; In situ; Corn silage.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162003000300007
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The Underwater Vision Profiler 5: An advanced instrument for high spatial resolution studies of particle size spectra and zooplankton ArchiMer
Picheral, Marc; Guidi, Lionel; Stemmann, Lars; Karl, David M.; Iddaoud, Ghizlaine; Gorsky, Gabriel.
The Underwater Vision Profiler (UVP) was developed to quantify the vertical distribution of macroscopic particles and zooplankton > 100 mu m in size. The smaller size limit is fixed by optical resolution, whereas the larger size limit is determined by the volume of water illuminated per image. The new fifth generation instrument (UVP5) is compact (30 kg in air) and operates either as a stand-alone instrument with an independent power supply for use on a mooring or free-drifting array, or as a component of a Conductivity, Temperature, and Depth (CTD)-rosette package. Images are recorded at a frequency up to 6 Hz. If the UVP5 is interfaced with a CTD, these images are acquired and analyzed in real time. Images are recorded every 20 cm at the 1 m s(-1)...
Tipo: Text Palavras-chave: Video plankton recorder; Marine snow; MU-M; Vertical distribution; Aggregate dynamics; In situ; System; Ocean; Flux; Phytoplankton.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00133/24413/22426.pdf
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Toward Detection of Harmful Algae Blooms by in situ Surface Plasmon Resonance Spectroscopy ArchiMer
Laurent, Sebastien; Colas, Florent; Hamelin, Muriel; Crassous, Marie-pierre; Antoine, Elisabeth; Lehaitre, Michel; Compere, Chantal.
Among marine algae species, Alexandrium minutum produces a phycotoxin called paralytic shellfish poisoning (PSP) that is introduced in the food chain through the ingestion of phytoplankton by shellfishs, and later by human consumers. Thus, in situ monitoring of A. minutum proliferation in coastal seawater is of great economical importance for marine resources exploitation. Here, we propose a rapid test for the detection of A. minutum by surface plasmon resonance spectroscopy. First, whole genomic DNA is extracted from the algae. Second, a 677 bp long portion of the 28S ribosomal DNA is amplified by PCR. Third, the PCR product is detected by surface plasmon resonance spectroscopy onto a DNA functionalized gold substrate.
Tipo: Text Palavras-chave: PCR; Algal detection; In situ; Spectroscopy; Surface plasmon resonance.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-6830.pdf
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Towards in situ detection of PAH trace in sea-water using SERS-active sensors ArchiMer
Peron, Olivier; Rinnert, Emmanuel; Lehaitre, Michel; Colas, Florent; Compere, Chantal.
This paper reports the development of a sensor based on surface-enhanced Raman scattering (SERS) for analyses in sea-water. Polycyclic aromatic hydrocarbons (PAHs) are targeted by these sensors and their detection in situ summons up chemical synthesis and optical development. Firstly, a relevant synthesis of SERS active substrates based on gold nanostructures is presented. Different kinds of substrates have been synthesized under variable experimental conditions to modify some parameters such as i) gold shape, size and distribution and such as ii) chemical functionalization: (i) gold nanoparticles were prepared either by chemical reduction of HAuCl4 or by physical deposition. (ii) Substrates were functionalized by hydrophobic films to allow nonpolar...
Tipo: Text Palavras-chave: Functionalization; Silanization; Gold nanostructures; SERS; PAH detection; Sea-water; Sensor; In situ.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/00012/12363/9152.pdf
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